Today all necessary chemicals arrived, so I started to synthesize the ONE TOUCH ULTRA control solution. I used:
- Deionized water
- Polyvinylpyrrolidone
- Sodium benzoate (min. 99%, Ph. Eur., BP, FCC, LM)
- EDTA (disodium salt)
- D(+)-Glucose (chemically pure)
- Indigo carmine (I was not able to purchase Amaranth)
Firstly I prepared a solution of 91ml deionized water, 8g polyvinylpyrrolidone, 0.5g sodium benzoate and 0.5g EDTA (disodium salt). As the EDTA (disodium salt) did not dissolve completely under room temperature I heated the solution up to approx. 50°C and stirred it frequently.
I added 0.38g glucose more to the solution. Now I got a proper reading: 135mg/dl, same as the original control solution.
Summarized: A control solution similar to the ONE TOUCH ULTRA one can be prepared by dissolving 8g polyvinylpyrrolidone, 0.5g sodium benzoate, 0.5g EDTA (disodium salt), 0.5g D(+)-Glucose and additionally a pinch of indigo carmine in 91ml deionized water.
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Great work. Control solution do seem to be a real pain. Commercial solutions seem to only come in 1-2 concentrations for each brand of strip. Also formulation of control solutions seems to be dependent on the strip chemistry (some rely on GDH some on GO) and other variables like hematocrit and hydration status. I thought control solutions would be a non-issue and instead they are an issue (and one I'm having to face now). So, again, Great Work.
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