In the above plot, is the reference spectrum from the Oregon Medical Laser Center's scan of Rhodamine B in Ethanol (6/20/1995,) and the scan I did on 10/1/2016, for the same substance (Rhodamine B in Ethanol).
The DH4 v4A Spectrometer's slit width is 0.12mm giving a spectral bandwidth of 1.6nm. Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.
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