12/19/2016; First serious calibration of the alignment and focus between the mirror, focal lens assembly and detector. I used a Quartz cuvette (clear on all 4 sides) this time, which has a wavelength range of 220nm - 2200nm and my Aries green 532nm DPSS high power laser (150mW,) I used a 99.99 % lab grade purity solution of Isopropyl alcohol in the quartz cuvette because its refractive index is 1.3772, so I would have only a crisp clear image of the laser line (spectra has been "normalized"):

References;

http://www.analiticaweb.com.br/newsletter/02/AN51721_UV.pdf

http://clinchem.aaccjnls.org/content/clinchem/21/11/1582.full.pdf