Yesterday I finally ordered the new PCB. This PCB is sponsored by the kind people of PCBWay, the well-known high-quality PCB fabrication company from China. During production you can always check in which production state your pcb is, and I watched an impressive series of videos that show all steps in the production process.

This is the KiCad 3D-impression of the new PCB:

With respect to the first PCB, the main changes are:

 - fix mistakes (VGA connector)
 - add Mouse connector
 - add HW for sending info to keyboard (and mouse)
 - add memory bank select
 - change video pixel/color generation for 640x400 mode
 - change WiFi module type
 - simple 4-wire connect to Raspberry Pi (for file transfer)
 - instead of a SPI header, a micro-SD card connector is mounted
 - changed SPI reading, it can now be done in 2 cycles/bit (instead of 5)