Okay Okay Okay 

so I need to slow down a little 

the plan is still 

1. grow E.coli

2.express gene in E.coli

3.do same thing in agrobacterium 

4.culture plants 

5.modify plants 

now I'm making a list for steps one and two focusing on one so now I need the BARE basics so what do I need to grow E.coli?

I have a list currently but hackaday is stupid and I can't upload files so I'm just gonna copy paste 

Okay so I just need something to sanitize them something to grow it in and something to freeze them in 

Just those steps 

I'm going to make a new list for the tools required just to do those  

So from this list I have the Incubator and the petri dishes 

Of course I will be needing other reagents but those are the most important 

The plan is to use a bunsen burner to generate a aseptic area for culturing cells in the beginning just for startup but 

But I gotta stop procrastinating I don't know why but my brain just want to push it away as hard as it can no matter what I wish I knew why

Now for the reagent LB broth is Extremely cheap to mix your own and glycerol is also extremely cheap 

The biggest cost here is the autoclave and micropipettes I need to get those down as those will be important now and In the future 

I need to get something done 

Also I found a new discovery while fishing for treasure in the garage a old food heater the kind that was meant for keeping things warm for long periods of time and it could possible be used as a new incubator  

It would work and is made out of metal and glass so it would look good too but who am I kidding I need to stop wasting my time and getting something done already

School has just been sucking up all of my time 

Right now I need help picking an autoclave this is very important as having an aseptic working area and technique is key to making any of this work 

I need help right now in picking an Autoclave as It will be key I might just settle for a pressure cooker right now and spend a little more money later on for a slightly nicer one once i've made some friends 

That's it for now I will give another update soon 

Peace