Home Biology Lab

So I made some slight changes to my original plan including some new goals on my way

Nothing major but things I will definitely be needing in the future hopefully. Also I was wondering if you guys could help pitch in with what I could use the lab for. I already have some ideas like growing neurons. As well as growing muscles and bones and maybe throwing together a little creature that can move of sorts. That future me's problem to deal with for now, I just think that having a clear goal in mind would definitely help majorly in terms of  having a visions and the direction I want to move my lab in. I'm probably gonna start cataloging all the junk in the garage so I can see what i wanna keep and what's heading to the garage sale. hopefully once i've cleared a space and gotten some extra cash from the sale I can start planning out for the lab and setting a plan into motion.

I've already started doing some research into Bio-safety cabinets and fume hoods I might separate the systemy into 2 parts the filter and the workstation. That way I can focus on each separately, also detaching it would man that if I decided to make a change to either I could do it with relative ease. A plexiglass and aluminum cage with a hole at the top may be all I need on the cage side. For the filter pulling air through a carbon fiber + HEPA filter should be adequate, I may do a couple different layers should it be needed. 

I plan on following the thought emporiums guide (AMAZING YOUTUBE CHANNEL )  when making the incubator but adjusting a reptile egg incubator. I do want to buy the centrifuge for safety reasons, and a pressure cooker should be sufficient for a autoclave for now until i need to size up or if we start having contamination problems. The inverted microscope has to be bought but I can try to make a fluorescent microscope. The water baths are cheap and easily available, Pipettes must be bought. I already own a freezer and fridge. The liquid nitrogen could be intresting if i choose to diy the tank but I probably won't. A controlled rate cooler is going to be very fun to make, same goes for the orbital shaker. And from their the sky's the limit.

The goal of this project is to set up a lab in the garage for all my cell culture needs ultimately aiming for mammalian cell culture and building my lab around that but I don't want to jump the gun. I went ahead and made a roadmap for all the tool i'll need.

The start of my goal is making a space where I can do my cultures and from there diverging into different tools depending on what opportunities are available to me at the time. Now I do own a 3d printer and some tools I do have a little experience with Arduino. I also plan on staying on a budget and doing everything for as cheap as I can hopefully with certain exceptions. That's about it for now i plan on doing a garage sale of the majority of the junk in the garage so hopefully that will increase the budget a little. can't wait to hear all the amazing ideas that come from here.