The Arduino code uses the serial monitor to take the following input. 

Denaturation Stage Temperature (C)

Denaturation Stage Duration (sec)

Annealing Stage Temperature (C)

Annealing Stage Duration (sec)

Extension Stage Temperature (C)

Extension Stage Duration (sec)

 Number of Cycles

Once these values re entered the process begins. The system is first raised to the Denaturation Stage temperature and held at this temperature for 120 seconds. The the Denaturation, Annealing and Extension stages are executed and repeated for the number of cycles entered.