Standardized open source DNA vectors

Updated my website with additional information. Will be posting soon on planned tests and updates on pKG basic vectors.

Did an overview of work it would take for a 'hello world' plant experiment. Going to begin learning more about specific plan tissue culture to perhaps make a compatible series of plasmids.

I have several yeast plasmids I would like to get working with soon. Will make compatible plasmids and reclone them.

Good progress, pKGs3 synthesis has finished and I am going to clone it next week. Hopefully I will be able to start adding parts by the end of May.

Interesting few weeks. As a preface-

pKGs2-sacB7_RFP is a plasmid for cloning vector parts. These vector parts can be used in a modular fashion to create new vectors. More information on this vector can be found on my website. Soon it will be updated to have viral(M13) packaging compatibility.

pKGs1 is the base vector. Parts can be put in this vector for storage before use in other contexts. It has a few advantages over pKGs2-sacB7_RFP in that it has superior transcriptional isolation, better primer binding sites, and a form of the T7 terminator at the 3' end.

I had some issues with pKGs2-sacB7_RFP stability, but it appears this was just because of old cam plates. Plasmid loss was rather slow, but still occurred. Perhaps it was because of the media, which was autoclaved with glucose. Anyway, it appears to be a stable plasmid. Now to just modify it with viral packaging! Updated log with files. Open with SnapGene viewer ( http://www.snapgene.com/ ).