DAV 4 UV/VIS Spectrometer

This is a CFL calibration done today using Spekwin32 control prototype capture program, and this is a test run for the ELP-USBFHD01M-l21 CMOS camera placed in it's mounting enclosure;

This is the new prototype CMOS Camera module mount, for the DAV 5 UV/VIS Spectrometer which I am now starting to bring back to life. I designed the camera mount using FreeCad version 0.16 and sculpted a working model out of high density matte foam.

400nm UV LED 2D EEM Plot

410nm UV high pwr 1W LED 4.0vdc 350mA-Prolight

470nm Blue high pwr 1W LED 4.0vdc 550mA-Cree

623nm Red high pwr 1W LED 2.7vdc 700mA-Cree

400nm UV thru-the-hole LED 40mA 9vdc-Generic

http://www.superbrightleds.com/?gclid=CKrPyo_GkcsCFQIfhgodD1gCsQ

410nm UV 1W LED 2D EEM Plot

470nm Blue LED 1W 2D EEM Plot

623nm RED LED 1W 2D EEM Plot

This is resolution test number 2 for my spectrometer after adjusting the DVD grating, I scanned a sample of Fluorescein in Ethanol and compared it to that of the standard done by the Oregon Medical Laser Center for the same sample: I have a quantum yield of 62.8 and a peak wavelength of 540nm, which hits the mark at the second peak on the OMLC's plot.

I did another resolution test for the DH 4.2 spectrometer today because I made some minor adjustments to the DVD grating and got a really good calibration with a quality fit of 7. ( the middle BLUE band is at 438nm ( 3nm over specs/perfection would be 435nm,) and the GREEN band is at a near perfect 547nm ( 1nm over spec.)

So I am just tickled pink!!

Well I finally got around to doing a current cost analysis for this project and I was pleased to see I was only .86 cents over budget!

My initial goal was to build a reliable and accurate homemade spectrometer, with professional industry standard resolution...and I'm getting ohhhh so close!!

Top View and Front View of PWR supply

I made a new power supply for my LEDs, and wanted to test it out so here are the results of that test:

Power supply: Old Zip drive/ITE/ Input-120vac 60Hz 0.3A

                                      Output- 5vdc 1A

Buck puck- LED driver module - 5-14vdc input 1000mA

400nm UV high pwr LED 3.5vdc 350mA/ Prolight 470nm Blue high pwr LED 3.5vdc 700mA/ Cree 623nm Red high pwr LED 2.7vdc 700mA/ Cree

I posted up some of the equipment that I use to get such precise concentration measurements when I am working with my spectrometer, well just click the picture and you'll find out!!

Well, I am within 5nm of the SpexFluoroMax spectrometer that the Oregon Medical Laser Center used for their spectroscopy studies. I am presenting my results here first, so everyone has a chance to soak in the value of a very simple and cost effective design verses a $25,000.00 piece of equipment...

Remember...within 5nm!!

I was trying to do something serious this morning, I had to realign the DVD grating in the spectrometer and my cat tee tee had other plans...

I think I got it right, although for some reason sample 4 has an anomaly ...Hmmm...