DAV5 V2.1 Spectrometer

Cuvette holder design and exit slit platform for the DAV5 V2 spectrometer

I am redesigning the DH4 V4a because I am having major problems with the ELP cmos camera module for the DAV5 V2, I will be incorperating many aspects into the DH4 V4a that are from the DAV5 spectrometer, mainly because the JDEPC-ov05 camera has proved to be far more reliable than I expected.

It also works just fine with the new upgrade of spekwin32 processing software (which is now SpectraGryph 1.0.) This design lets the camera module nestle snugly in the inlay of the holder and allows a 3mm distance between the DVD diffraction grating and camera eye lens.

This design will be far more sophisticated than anything I have built so far, because this will be a truer spectrometer in the sense that it will have a reflective mirror and concave focusing mirror that will direct the diffracted spectrum to the detector for a sharper and higher resolution spectrum.

The new cost contraints will be under 150.00 US dollars, still well under anything out there with this kind of performance. The materials will be sourced from mostly local hardware stores when possible.

The first plot is a Solux 12vdc 50W 4700K Reference spectrum I downloaded from the Solux website, under their technical specifications section, so I could reference it against my Solux lamp of the same specs.

I modified the camera holder for the DH 4 V4a spectrometer (the one with the JDEPC-ov05 cmos camera,) and used the prototype design from the DAV5 blueprints for the ELP cmos camera mount and sculpted a new camera mount, that now, not only houses the camera but securely fastened the DVD grating to the mount itself for perfect alignment.

I also wanted to experiment with a polarizing filter I have (a big one at; 82mm dia.) The effect is very prominent.

I finally figured out what that "spike" is at 433nm, I inspected the camera with the lens off, and found a scratch underneath the focusing lens, it must have happened during my removal of the IR filter! :(

This procedure is unique in the sense that it is called "spiking the sample," an initial baseline scan is run and without interruption of laser excitation, the sample is "spiked" with the Acetic acid at timed intervals like a camera taking multiple snapshots.

Absorption and Emission plot for Rhodamine B in Ethanol, using a Solux 4700K 50W 12vdc lamp for absorption measurements and a 150mW 532nm Green laser for fluorescence measurements;

Next, is the absorption plot for all 3 Rhodamine B scans (raw data,) scan 3,4 and 5. Scan 3 was chosen because it had the closest molar absorption coefficient match to the reference spectrum (OMLC,Oregon Medical Laser Center's scan of the same compound-Rhodamine B in Ethanol.)

In the above plot, is the reference spectrum from the Oregon Medical Laser Center's scan of Rhodamine B in Ethanol (6/20/1995,) and the scan I did on 10/1/2016, for the same substance (Rhodamine B in Ethanol).

The DH4 v4A Spectrometer's slit width is 0.12mm giving a spectral bandwidth of 1.6nm. Samples were prepared in 1cm pathlength quartz cells with absorbance less than 0.1 at the excitation and all emission wavelengths to uniformly illuminate across the sample, and to avoid the inner-filter effect. The dark counts were subtracted and the spectra were corrected for wavelength-dependent instrument sensitivity.

References:

http://www.ebay.com/itm/like/Jobin-Yvon-Horiba-ISA-Spex-FluoroMax-2-200-900nm-Spectrofluorometer/262477107822

http://www.bio.huji.ac.il/upload/Optical_Spectrofluorometers_Manuals.pdf

http://omlc.org/spectra/PhotochemCAD/html/061.html

These are the 7 major dyes I work with:

2 compounds I use to adjust for pH levels:

These are the solvents that I work with, I included there respective Raman bands, because this is the data that I use to remove Raman/Raleigh scatter.