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TOTAL COST RANGES FROM $400 TO 900
Depending on how DIY you go!
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Grasshoppers
FREE - As many as you can catch!
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iPad
~$400 or if already have - As screen for visual stimuli and recording and visualizing spikes, in a cumulative SpikeRecorder app
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Microscope
$200 or ask around or use a phone app! - 20X magnification is sufficient; For surgery on grasshopper and electrode placement
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Magnetic stirrer
$100 or be creative! -To heat, melt and mix the wax and rosin mixture
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RadioShack mini speaker/amplifier
$15 - To hear the spikes when the grasshopper sees the balls being thrown at it
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Backyard Brains Neuron SpikerBox
$100 or DIY - A bioamplifier that allows us to hear and see spikes in living neurons; has cork board piece on top for mounting animal
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Backyard Brains Micromanipulator
$100 or DIY - For precise placement of electrodes
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1-Channel Electrodes: Reference and Recording
included with manipulator or DIY - Hook electrode around neck connective of grasshopper and reference electrode in abdomen, both connected to SpikerBox
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Painter's Tape
$2 - To restrain grasshopper and tape it down to corkboard piece on the SpikerBox
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Thread
$2 - To pull grasshopper's neck up to expose neck where recording electrode is placed
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Wax and violin rosin mixture
$6 - Heated to be mixed together; for placing on thread around grasshopper's neck to keep it in place
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