Coffee Cup Polymerase Chain Reaction Machine

The Arduino code uses the serial monitor to take the following input. 

Denaturation Stage Temperature (C)

Denaturation Stage Duration (sec)

Annealing Stage Temperature (C)

Annealing Stage Duration (sec)

Extension Stage Temperature (C)

Extension Stage Duration (sec)

 Number of Cycles

Once these values re entered the process begins. The system is first raised to the Denaturation Stage temperature and held at this temperature for 120 seconds. The the Denaturation, Annealing and Extension stages are executed and repeated for the number of cycles entered.

The current code, CCPCRv1, runs the following fixed routine. 

0. Raise temp to 95C

1. Stay at 95C for 120 seconds

2. Stay at 95C for 15 seconds

3. Drop temp to 55C

4. Stay at 55C for 15 seconds

5. Raise temp to 68C

6. Stay at 68C for 45 seconds

7. Raise temp to 95C

*8. Repeat steps 2 through 17 nineteen times. *(Not Implemented yet)

9. End

Use the Arduino IDE serial port monitor to watch progress.

Hot glue the fan to the plywood top directly over the center hole.

Then wire the components based on the sketch below.

1. Cut a small 1/4" piece plywood into a 4 1/2" Square

2. Cut holes as shown in sketch