DAV 4 UV/VIS Spectrometer

Details

I wanted to talk a little about why I started this project and how it has evolved into something unexpected in such a short time. I bought this kit out of trying to help a friend out who had a sample that they wanted tested to find out what it was (they really just didn't want to spend about 900.00 to take it to a lab and test it, but hey, what are friends for!)

So anyways, I said well let me see what I can come up with and I saw this spectrometer kit and said hey I bet I could take that kit and hype it up to analyze this stuff and see what it is, well it was ruff waters at first, since I had NO experience with spectrometry, only engineering and chemistry, but I never give up and I just kept studying and tinkering and modifying until walla, Plab 2.5 (build 3.0.1 was born!)

I did find out what the sample was (Salicyclic acid with an anti-biotic mixed in...it was in a container where it should not have been...hummm.)

I am getting a new cmos sensor next week, so I'll document that and post up the results, it's a far superior camera module and has better sensitivity, plus I upgraded the DVD design to be even more stable so that will be another great improvement.

The biggest obstacle I have had to overcome to get quality results is stability and alignment of the hardware is very critical!!

Files

top view elp.png

Top view.

Portable Network Graphics (PNG) - 631.92 kB - 10/12/2016 at 12:44

Preview

side view elp.png

Side view.

Portable Network Graphics (PNG) - 626.63 kB - 10/12/2016 at 12:43

Preview

front view elp.png

Front view of camera mount.

Portable Network Graphics (PNG) - 554.31 kB - 10/12/2016 at 12:41

Preview

bottom view elp.png

Portable Network Graphics (PNG) - 681.03 kB - 10/12/2016 at 12:40

Preview

close up of back.png

Portable Network Graphics (PNG) - 1.98 MB - 05/03/2016 at 21:53

Preview

Components

1 × DVD R/W 18 X 18mm Sq. 8.5 Gigabyte used for diffraction grating

1 × ELP-USBFHD01M-L21 Cmos module-Cost $45.00 (amazon.com) CMOS 1080P sensor for high quality image and low power consumption.

1 × non-compressible yoga block (high density black foam) very versatile, can be cut very precisely and is non-reflective.

1 × High Performance Black Masking Tape 2" T743-2.0 ThorLabs Inc.,

1 × Gillette Razor Blade open-air entrance slit 0.18mm slit width (entrance for diffraction grating)

Project Logs

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Test Run for CMOS camera
David H Haffner Sr • 10/16/2016 at 21:06 • 0 comments

This is a CFL calibration done today using Spekwin32 control prototype capture program, and this is a test run for the ELP-USBFHD01M-l21 CMOS camera placed in it's mounting enclosure;
DAV 5 UV/VIS Spectrometer, CMOS Camera Mount Design
David H Haffner Sr • 10/13/2016 at 13:23 • 0 comments

This is the new prototype CMOS Camera module mount, for the DAV 5 UV/VIS Spectrometer which I am now starting to bring back to life. I designed the camera mount using FreeCad version 0.16 and sculpted a working model out of high density matte foam.
Final Resolution Test for DH 4.2 Homemade Spectrometer
David H Haffner Sr • 06/20/2016 at 09:02 • 0 comments

400nm UV LED 2D EEM Plot
I tested these 4 LEDs at the highest gain possible, by scanning them straight ahead through the detector's slit with no filter thru an empty glass cuvette. This way I can get a better reading on their true values as compared to their stated values.
410nm UV high pwr 1W LED 4.0vdc 350mA-Prolight
470nm Blue high pwr 1W LED 4.0vdc 550mA-Cree
623nm Red high pwr 1W LED 2.7vdc 700mA-Cree
400nm UV thru-the-hole LED 40mA 9vdc-Generic
http://www.superbrightleds.com/?gclid=CKrPyo_GkcsCFQIfhgodD1gCsQ
410nm UV 1W LED 2D EEM Plot
470nm Blue LED 1W 2D EEM Plot
623nm RED LED 1W 2D EEM Plot
Resolution Test number 2 DH 4.2 Spectrometer Fluorescein
David H Haffner Sr • 06/19/2016 at 12:52 • 0 comments

This is resolution test number 2 for my spectrometer after adjusting the DVD grating, I scanned a sample of Fluorescein in Ethanol and compared it to that of the standard done by the Oregon Medical Laser Center for the same sample: I have a quantum yield of 62.8 and a peak wavelength of 540nm, which hits the mark at the second peak on the OMLC's plot.
Resolution Test using CFL light source
David H Haffner Sr • 06/18/2016 at 23:50 • 0 comments

I did another resolution test for the DH 4.2 spectrometer today because I made some minor adjustments to the DVD grating and got a really good calibration with a quality fit of 7. ( the middle BLUE band is at 438nm ( 3nm over specs/perfection would be 435nm,) and the GREEN band is at a near perfect 547nm ( 1nm over spec.)
So I am just tickled pink!!
Cost analysis for the DH 4.2 spectrometer (homemade)
David H Haffner Sr • 06/17/2016 at 09:59 • 0 comments

Well I finally got around to doing a current cost analysis for this project and I was pleased to see I was only .86 cents over budget!
My initial goal was to build a reliable and accurate homemade spectrometer, with professional industry standard resolution...and I'm getting ohhhh so close!!
Tested 3 High Powered LEDs Red, Blue and Violet (UV)
David H Haffner Sr • 06/11/2016 at 17:13 • 0 comments
Top View and Front View of PWR supply
I made a new power supply for my LEDs, and wanted to test it out so here are the results of that test:
Power supply: Old Zip drive/ITE/ Input-120vac 60Hz 0.3A
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                                      Output- 5vdc 1A
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Buck puck- LED driver module - 5-14vdc input 1000mA
400nm UV high pwr LED 3.5vdc 350mA/ Prolight 470nm Blue high pwr LED 3.5vdc 700mA/ Cree 623nm Red high pwr LED 2.7vdc 700mA/ Cree
In Case Anyone Was Wondering...:)
David H Haffner Sr • 06/06/2016 at 14:12 • 0 comments

I posted up some of the equipment that I use to get such precise concentration measurements when I am working with my spectrometer, well just click the picture and you'll find out!!
DH 4.2 Spectrometer Calibration June 4th
David H Haffner Sr • 06/04/2016 at 12:48 • 0 comments

Well, I am within 5nm of the SpexFluoroMax spectrometer that the Oregon Medical Laser Center used for their spectroscopy studies. I am presenting my results here first, so everyone has a chance to soak in the value of a very simple and cost effective design verses a $25,000.00 piece of equipment...
Remember...within 5nm!!
My cat helped me out on this one!
David H Haffner Sr • 06/01/2016 at 17:06 • 0 comments

I was trying to do something serious this morning, I had to realign the DVD grating in the spectrometer and my cat tee tee had other plans...
I think I got it right, although for some reason sample 4 has an anomaly ...Hmmm...

View all 26 project logs

Build Instructions

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Step 1

These are the initial instructions for building the version 2.5 spectrometer, from these and with much pratical application and modifications the v2.5 (build 3.0.1) was born.

Step 2

This is the new proto type CMOS Camera module mount, for the DAV 5 UV/VIS Spectrometer which I am now starting to bring back to life. I designed the camera mount using FreeCad version 0.16 and sculpted a working model out of high density matte foam.

REV.1

Step 3

This is my new Gillette razor blade open-air slit design; 0.12mm slit width. Verified using my Aries DPSS 150mW 532nm Green Laser through a quartz cuvette containing 99.99% purity Isopropyl alcohol (lab grade certified.)

Discussions

Dr. Cockroach wrote 08/21/2017 at 13:00

Looking good my friend, way beyond me but it all looks great :-)

Are you sure? yes | no

David H Haffner Sr wrote 08/21/2017 at 16:49

Hey DOC, yeah this was the 2nd evolutionary step to my current project :)

Are you sure? yes | no

David H Haffner Sr wrote 08/21/2016 at 15:00

Hey Jong Kim, yeah the high powered laser is a must, for the raman spectrometry because of its stability, but the 5mW will work, I have used it.

Are you sure? yes | no

David H Haffner Sr wrote 08/19/2016 at 07:01

Hey Jong Kim, yeah, that;s my Aries 150mW green Portable laser (532nm) it is a DPSS type, here is where I got it from http://www.laserglow.com/GAR

Dave H

Are you sure? yes | no

Jong KIM wrote 08/21/2016 at 12:55

Hi, David Haffner Sr.

Thank you for your kind reply.
A few days ago, I've got a low-cost green laser module with 5mW power.
I guess, for the Raman spectrometer design, a high powered laser like you have selected is a must.

Are you sure? yes | no

Jong KIM wrote 08/19/2016 at 05:59

Hi, David.

Could you possibly give me some info on your 2nd image, next to the new camera module you employed in this project, which to me looks like a laser module or laser pointer.

Thanks,

Jong

Are you sure? yes | no

David H Haffner Sr wrote 07/05/2016 at 08:08

Thanks Jong KIM, it's stll a ways from begin a Raman instrument but it's operating specs as of right now are surprising even me.

Are you sure? yes | no

Jong KIM wrote 07/05/2016 at 05:20

Quite superb result!

Your simple yet powerful spectrometer is aiming toward the analysis by Raman spectrophotometry !

Are you sure? yes | no

DAV 4 UV/VIS Spectrometer

Description

Details